Michael G. Rosenfeld, UCSD
"Coactivator and Corepressor Networks Integrated Transcriptional Response Programs"
Transcriptional regulation is done by exchange of activators and co-repressors on the binding sites.
Uses a variation of chip-chip called chip-dsl-chip where they biotinynlate the RNA and use 40 mer primers to ligate small fragments that can be detected on a chip (this avoids the random sizes of sonication problem in traditional chip-chip)
Most profound in this talk is the notion that there is some interplay between the transcriptional activation machinery and PARP1 which is known to be involved in NDA-damage response. This led to the idea that there is a DNA double strand nick that is associated with transcriptional activation caused by TopoIIb.
posted by The Bioinformatics Blog @ 11:43 AM
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